The observation of fluorescence in a tissue offers a method of real-time monitoring of chemical reactions when one of the reagents or products in fluorescent. This paper discusses how tissue optics affects one's observation of fluorescence in a turbid medium such as tissue. An experimental example is also shown: the photobleaching of a photosensitizer molecule (protoporphyrin IX) within rat tumors. A simple algorithm (Gardner et al., 1993) is given which enables the corrections for tissue optics.
S. L. Jacques, "Optical monitoring of chemical reactions by fluorescence in turbid media," SPIE Proceedings of Laser- Tissue Interaction IV, edited by S. L. Jacques, A. Katzir, 1882, 73-83 (1993).