The molar extinction coefficient of Nile Red dissolved in Dioxane based on the absorbance measurements made by R. A. Fuh on 6/16/95 using a Cary 3 [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. The absorbance data was normalized to have an extinction coefficient of 38000 at 519.4 nm [M. M. Davis and H. B. Hetzer, "Titrimetric and equilibrium studies using indicators related to Nile Blue A," Anal. Chem., 38, 451-461, 1966].

To convert this data to absorbance A=-log10(T), multiply by the molar concentration and the pathlength in centimeters. To convert this data to absorption coefficient in (cm-1), multiply by the molar concentration and 2.303.

lambda   	Extinction 
nm       	cm-1/M

 249.72	   23446
 250.00	   24258
 250.28	   24653
 250.56	   25029
 250.83	   25636
 251.11	   26112
 251.39	   26510
 251.67	   26739
 251.94	   27353
 252.22	   27682
 252.50	   28050
 252.78	   28489
 253.06	   28695
 253.33	   28976
 253.61	   28993
 253.89	   28757
 254.17	   28988
 254.44	   29059
 254.72	   29284
 255.00	   29760
 255.28	   30027
 255.56	   30435
 255.83	   31213
 256.11	   31825
 256.39	   32571
 256.67	   33011
 256.94	   33772
 257.22	   34422
 257.50	   34909
 257.78	   35592
 258.06	   36150
 258.33	   36727
 258.61	   37254
 258.89	   37930
 259.17	   38426
 259.44	   38707
 259.72	   39097
 260.00	   39136
 260.28	   39237
 260.56	   39376
 260.83	   39649
 261.11	   39750
 261.39	   39925
 261.67	   39853
 261.94	   39939
 262.22	   39954
 262.50	   39638
 262.78	   39222
 263.06	   38783
 263.33	   37928
 263.61	   37079
 263.89	   36309
 264.17	   35158
 264.44	   34135
 264.72	   33025
 265.00	   31744
 265.28	   30579
 265.56	   29309
 265.83	   28207
 266.11	   26978
 266.39	   25742
 266.67	   24557
 266.94	   23527
 267.22	   22377
 267.50	   21325
 267.78	   20443
 268.06	   19472
 268.33	   18605
 268.61	   17748
 268.89	   17067
 269.17	   16299
 269.44	   15672
 269.72	   15070
 270.00	   14482
 270.28	   14003
 270.56	   13510
 270.83	   13132
 271.11	   12643
 271.39	   12369
 271.67	   11936
 271.94	   11731
 272.22	   11291
 272.50	   11001
 272.78	   10830
 273.06	   10587
 273.33	   10329
 273.61	   10130
 273.89	    9901
 274.17	    9729
 274.44	    9595
 274.72	    9530
 275.00	    9385
 275.28	    9172
 275.56	    9047
 275.83	    8969
 276.11	    8769
 276.39	    8673
 276.67	    8604
 276.94	    8507
 277.22	    8463
 277.50	    8471
 277.78	    8352
 278.06	    8277
 278.33	    8171
 278.61	    8184
 278.89	    8064
 279.17	    8112
 279.44	    8022
 279.72	    7927
 280.00	    7917
 280.28	    7964
 280.56	    7886
 280.83	    7949
 281.11	    7819
 281.39	    7797
 281.67	    7678
 281.94	    7741
 282.22	    7705
 282.50	    7675
 282.78	    7730
 283.06	    7786
 283.33	    7662
 283.61	    7703
 283.89	    7723
 284.17	    7664
 284.44	    7690
 284.72	    7749
 285.00	    7650
 285.28	    7724
 285.56	    7789
 285.83	    7858
 286.11	    7756
 286.39	    7766
 286.67	    7914
 286.94	    7855
 287.22	    7888
 287.50	    7923
 287.78	    7956
 288.06	    7975
 288.33	    8064
 288.61	    8117
 288.89	    8016
 289.17	    8089
 289.44	    8048
 289.72	    8098
 290.00	    8150
 290.28	    8186
 290.56	    8220
 290.83	    8247
 291.11	    8263
 291.39	    8318
 291.67	    8398
 291.94	    8362
 292.22	    8501
 292.50	    8453
 292.78	    8354
 293.06	    8531
 293.33	    8500
 293.61	    8518
 293.89	    8530
 294.17	    8555
 294.44	    8557
 294.72	    8570
 295.00	    8570
 295.28	    8602
 295.56	    8642
 295.83	    8602
 296.11	    8692
 296.39	    8762
 296.67	    8718
 296.94	    8783
 297.22	    8817
 297.50	    8845
 297.78	    8798
 298.06	    8898
 298.33	    8930
 298.61	    8901
 298.89	    8934
 299.17	    9022
 299.44	    8972
 299.72	    9021
 300.00	    8998
 300.28	    8950
 300.56	    9046
 300.83	    8983
 301.11	    9026
 301.39	    9115
 301.67	    9088
 301.94	    9035
 302.22	    9022
 302.50	    8983
 302.78	    9046
 303.06	    8980
 303.33	    9017
 303.61	    8977
 303.89	    8966
 304.17	    8883
 304.44	    8962
 304.72	    8898
 305.00	    8865
 305.28	    8896
 305.56	    8783
 305.83	    8850
 306.11	    8729
 306.39	    8752
 306.67	    8660
 306.94	    8628
 307.22	    8538
 307.50	    8534
 307.78	    8524
 308.06	    8405
 308.33	    8426
 308.61	    8450
 308.89	    8274
 309.17	    8184
 309.44	    8151
 309.72	    8084
 310.00	    8114
 310.28	    7968
 310.56	    7963
 310.83	    7784
 311.11	    7683
 311.39	    7798
 311.67	    7673
 311.94	    7592
 312.22	    7511
 312.50	    7543
 312.78	    7521
 313.06	    7434
 313.33	    7364
 313.61	    7414
 313.89	    7608
 314.17	    7442
 314.44	    7083
 314.72	    7183
 315.00	    7111
 315.28	    7201
 315.56	    7200
 315.83	    7234
 316.11	    7133
 316.39	    7004
 316.67	    7133
 316.94	    7056
 317.22	    7046
 317.50	    7261
 317.78	    7036
 318.06	    6924
 318.33	    7123
 318.61	    7093
 318.89	    6949
 319.17	    6752
 319.44	    6622
 319.72	    7077
 320.00	    6970
 320.28	    6858
 320.56	    6859
 320.83	    6801
 321.11	    6818
 321.39	    6640
 321.67	    6790
 321.94	    6699
 322.22	    6881
 322.50	    6537
 322.78	    6743
 323.06	    6589
 323.33	    6686
 323.61	    6431
 323.89	    6292
 324.17	    6379
 324.44	    6286
 324.72	    6101
 325.00	    6264
 325.28	    6125
 325.56	    6266
 325.83	    6045
 326.11	    6152
 326.39	    5964
 326.67	    5818
 326.94	    6133
 327.22	    5725
 327.50	    5756
 327.78	    5724
 328.06	    5765
 328.33	    5622
 328.61	    5697
 328.89	    5741
 329.17	    5575
 329.44	    5533
 329.72	    5459
 330.00	    5421
 330.28	    5437
 330.56	    5394
 330.83	    5485
 331.11	    5464
 331.39	    5389
 331.67	    5065
 331.94	    5226
 332.22	    5238
 332.50	    4957
 332.78	    4763
 333.06	    4916
 333.33	    4714
 333.61	    4865
 333.89	    5006
 334.17	    4955
 334.44	    4682
 334.72	    4808
 335.00	    4912
 335.28	    4707
 335.56	    4739
 335.83	    4469
 336.11	    4629
 336.39	    4601
 336.67	    4463
 336.94	    4182
 337.22	    4512
 337.50	    4428
 337.78	    4292
 338.06	    4087
 338.33	    4268
 338.61	    3917
 338.89	    4027
 339.17	    4118
 339.44	    3905
 339.72	    4065
 340.00	    4070
 340.28	    3808
 340.56	    3761
 340.83	    3632
 341.11	    3518
 341.39	    3702
 341.67	    3549
 341.94	    3483
 342.22	    3474
 342.50	    3523
 342.78	    3480
 343.06	    3034
 343.33	    3202
 343.61	    3308
 343.89	    3247
 344.17	    3060
 344.44	    3045
 344.72	    3036
 345.00	    3033
 345.28	    3112
 345.56	    2766
 345.83	    2761
 346.11	    2967
 346.39	    2761
 346.67	    2616
 346.94	    2726
 347.22	    2632
 347.50	    2681
 347.78	    2540
 348.06	    2494
 348.33	    2517
 348.61	    2652
 348.89	    2352
 349.17	    2488
 349.44	    2382
 349.72	    2365
 350.00	    2327
 350.28	    2255
 350.56	    2375
 350.83	    2175
 351.11	    2163
 351.39	    2121
 351.67	    2121
 351.94	    2160
 352.22	    2168
 352.50	    2190
 352.78	    1959
 353.06	    2111
 353.33	    1990
 353.61	    2101
 353.89	    2152
 354.17	    2025
 354.44	    2059
 354.72	    2067
 355.00	    2106
 355.28	    1929
 355.56	    2067
 355.83	    1985
 356.11	    2066
 356.39	    1977
 356.67	    2011
 356.94	    1919
 357.22	    1926
 357.50	    1974
 357.78	    1995
 358.06	    1927
 358.33	    2059
 358.61	    1951
 358.89	    1847
 359.17	    1920
 359.44	    1924
 359.72	    1866
 360.00	    1733
 360.28	    1905
 360.56	    1844
 360.83	    1880
 361.11	    1810
 361.39	    1899
 361.67	    1902
 361.94	    1890
 362.22	    1766
 362.50	    1684
 362.78	    1796
 363.06	    1708
 363.33	    1656
 363.61	    1682
 363.89	    1625
 364.17	    1673
 364.44	    1588
 364.72	    1717
 365.00	    1715
 365.28	    1660
 365.56	    1666
 365.83	    1705
 366.11	    1655
 366.39	    1673
 366.67	    1564
 366.94	    1694
 367.22	    1656
 367.50	    1590
 367.78	    1560
 368.06	    1610
 368.33	    1564
 368.61	    1659
 368.89	    1610
 369.17	    1505
 369.44	    1652
 369.72	    1437
 370.00	    1567
 370.28	    1618
 370.56	    1561
 370.83	    1555
 371.11	    1595
 371.39	    1676
 371.67	    1655
 371.94	    1635
 372.22	    1537
 372.50	    1629
 372.78	    1574
 373.06	    1638
 373.33	    1703
 373.61	    1630
 373.89	    1586
 374.17	    1575
 374.44	    1629
 374.72	    1658
 375.00	    1669
 375.28	    1563
 375.56	    1703
 375.83	    1579
 376.11	    1599
 376.39	    1594
 376.67	    1569
 376.94	    1521
 377.22	    1679
 377.50	    1757
 377.78	    1680
 378.06	    1789
 378.33	    1660
 378.61	    1619
 378.89	    1591
 379.17	    1641
 379.44	    1722
 379.72	    1649
 380.00	    1602
 380.28	    1668
 380.56	    1652
 380.83	    1672
 381.11	    1672
 381.39	    1624
 381.67	    1572
 381.94	    1676
 382.22	    1708
 382.50	    1760
 382.78	    1712
 383.06	    1788
 383.33	    1741
 383.61	    1667
 383.89	    1712
 384.17	    1702
 384.44	    1721
 384.72	    1692
 385.00	    1661
 385.28	    1553
 385.56	    1728
 385.83	    1786
 386.11	    1736
 386.39	    1762
 386.67	    1734
 386.94	    1743
 387.22	    1789
 387.50	    1725
 387.78	    1774
 388.06	    1756
 388.33	    1702
 388.61	    1751
 388.89	    1774
 389.17	    1761
 389.44	    1812
 389.72	    1644
 390.00	    1759
 390.28	    1752
 390.56	    1786
 390.83	    1771
 391.11	    1884
 391.39	    1798
 391.67	    1836
 391.94	    1719
 392.22	    1812
 392.50	    1850
 392.78	    1894
 393.06	    1788
 393.33	    1704
 393.61	    1743
 393.89	    1874
 394.17	    1862
 394.44	    1838
 394.72	    1849
 395.00	    1728
 395.28	    1921
 395.56	    1785
 395.83	    1756
 396.11	    1862
 396.39	    1799
 396.67	    1892
 396.94	    1983
 397.22	    2015
 397.50	    1893
 397.78	    1958
 398.06	    1951
 398.33	    1936
 398.61	    1984
 398.89	    1907
 399.17	    1905
 399.44	    1953
 399.72	    1907
 400.00	    1960
 400.28	    2051
 400.56	    2093
 400.83	    1991
 401.11	    2039
 401.39	    2072
 401.67	    2132
 401.94	    1987
 402.22	    2005
 402.50	    2065
 402.78	    2027
 403.06	    2117
 403.33	    2124
 403.61	    2013
 403.89	    2154
 404.17	    2186
 404.44	    2167
 404.72	    2207
 405.00	    2114
 405.28	    2130
 405.56	    2156
 405.83	    2216
 406.11	    2239
 406.39	    2173
 406.67	    2292
 406.94	    2245
 407.22	    2250
 407.50	    2325
 407.78	    2245
 408.06	    2183
 408.33	    2381
 408.61	    2266
 408.89	    2268
 409.17	    2324
 409.44	    2372
 409.72	    2411
 410.00	    2421
 410.28	    2471
 410.56	    2535
 410.83	    2437
 411.11	    2503
 411.39	    2456
 411.67	    2499
 411.94	    2515
 412.22	    2573
 412.50	    2548
 412.78	    2589
 413.06	    2605
 413.33	    2681
 413.61	    2596
 413.89	    2718
 414.17	    2648
 414.44	    2705
 414.72	    2656
 415.00	    2682
 415.28	    2698
 415.56	    2789
 415.83	    2710
 416.11	    2871
 416.39	    2793
 416.67	    2914
 416.94	    2818
 417.22	    2958
 417.50	    2929
 417.78	    2919
 418.06	    2991
 418.33	    3018
 418.61	    3061
 418.89	    3136
 419.17	    3046
 419.44	    3162
 419.72	    3076
 420.00	    3255
 420.28	    3238
 420.56	    3240
 420.83	    3251
 421.11	    3341
 421.39	    3341
 421.67	    3341
 421.94	    3444
 422.22	    3416
 422.50	    3521
 422.78	    3489
 423.06	    3689
 423.33	    3520
 423.61	    3654
 423.89	    3619
 424.17	    3648
 424.44	    3683
 424.72	    3711
 425.00	    3822
 425.28	    3811
 425.56	    3901
 425.83	    3925
 426.11	    3967
 426.39	    4030
 426.67	    4017
 426.94	    4060
 427.22	    4113
 427.50	    4236
 427.78	    4152
 428.06	    4315
 428.33	    4276
 428.61	    4254
 428.89	    4435
 429.17	    4402
 429.44	    4481
 429.72	    4508
 430.00	    4529
 430.28	    4594
 430.56	    4728
 430.83	    4719
 431.11	    4768
 431.39	    4838
 431.67	    4879
 431.94	    4984
 432.22	    5021
 432.50	    5081
 432.78	    5157
 433.06	    5180
 433.33	    5278
 433.61	    5319
 433.89	    5363
 434.17	    5379
 434.44	    5466
 434.72	    5557
 435.00	    5596
 435.28	    5634
 435.56	    5715
 435.83	    5816
 436.11	    5811
 436.39	    5929
 436.67	    6039
 436.94	    6023
 437.22	    6066
 437.50	    6195
 437.78	    6300
 438.06	    6393
 438.33	    6437
 438.61	    6460
 438.89	    6480
 439.17	    6593
 439.44	    6657
 439.72	    6679
 440.00	    6694
 440.28	    6912
 440.56	    6992
 440.83	    7030
 441.11	    7026
 441.39	    7187
 441.67	    7257
 441.94	    7331
 442.22	    7366
 442.50	    7520
 442.78	    7496
 443.06	    7573
 443.33	    7700
 443.61	    7770
 443.89	    7923
 444.17	    7907
 444.44	    8074
 444.72	    8092
 445.00	    8235
 445.28	    8213
 445.56	    8375
 445.83	    8450
 446.11	    8447
 446.39	    8565
 446.67	    8672
 446.94	    8826
 447.22	    8904
 447.50	    8978
 447.78	    9047
 448.06	    9116
 448.33	    9308
 448.61	    9370
 448.89	    9460
 449.17	    9545
 449.44	    9654
 449.72	    9785
 450.00	    9846
 450.28	    9947
 450.56	   10050
 450.83	   10113
 451.11	   10250
 451.39	   10324
 451.67	   10441
 451.94	   10589
 452.22	   10632
 452.50	   10697
 452.78	   10855
 453.06	   11025
 453.33	   11099
 453.61	   11143
 453.89	   11270
 454.17	   11490
 454.44	   11526
 454.72	   11582
 455.00	   11568
 455.28	   11762
 455.56	   11880
 455.83	   11938
 456.11	   12139
 456.39	   12240
 456.67	   12346
 456.94	   12445
 457.22	   12685
 457.50	   12619
 457.78	   12780
 458.06	   12867
 458.33	   13054
 458.61	   13222
 458.89	   13318
 459.17	   13474
 459.44	   13585
 459.72	   13778
 460.00	   13878
 460.28	   13938
 460.56	   14110
 460.83	   14290
 461.11	   14357
 461.39	   14449
 461.67	   14678
 461.94	   14654
 462.22	   14852
 462.50	   15020
 462.78	   15147
 463.06	   15308
 463.33	   15498
 463.61	   15497
 463.89	   15713
 464.17	   15805
 464.44	   15995
 464.72	   16053
 465.00	   16263
 465.28	   16388
 465.56	   16530
 465.83	   16660
 466.11	   16761
 466.39	   16939
 466.67	   17059
 466.94	   17263
 467.22	   17308
 467.50	   17393
 467.78	   17558
 468.06	   17735
 468.33	   17976
 468.61	   17975
 468.89	   18282
 469.17	   18359
 469.44	   18440
 469.72	   18567
 470.00	   18703
 470.28	   18873
 470.56	   19060
 470.83	   19133
 471.11	   19286
 471.39	   19467
 471.67	   19733
 471.94	   19766
 472.22	   19813
 472.50	   20054
 472.78	   20314
 473.06	   20337
 473.33	   20498
 473.61	   20597
 473.89	   20775
 474.17	   20973
 474.44	   20945
 474.72	   21206
 475.00	   21369
 475.28	   21568
 475.56	   21717
 475.83	   21865
 476.11	   22105
 476.39	   22194
 476.67	   22326
 476.94	   22528
 477.22	   22751
 477.50	   22850
 477.78	   22944
 478.06	   23109
 478.33	   23244
 478.61	   23491
 478.89	   23490
 479.17	   23615
 479.44	   23773
 479.72	   24076
 480.00	   24139
 480.28	   24372
 480.56	   24438
 480.83	   24527
 481.11	   24740
 481.39	   24842
 481.67	   25010
 481.94	   25180
 482.22	   25345
 482.50	   25591
 482.78	   25645
 483.06	   25834
 483.33	   25914
 483.61	   26157
 483.89	   26266
 484.17	   26291
 484.44	   26569
 484.72	   26739
 485.00	   26880
 485.28	   27037
 485.56	   27154
 485.83	   27318
 486.11	   27454
 486.39	   27671
 486.67	   27781
 486.94	   28126
 487.22	   28133
 487.50	   28326
 487.78	   28333
 488.06	   28658
 488.33	   28823
 488.61	   28857
 488.89	   29163
 489.17	   29269
 489.44	   29371
 489.72	   29585
 490.00	   29730
 490.28	   29841
 490.56	   30001
 490.83	   30186
 491.11	   30404
 491.39	   30504
 491.67	   30696
 491.94	   30796
 492.22	   30899
 492.50	   31065
 492.78	   31227
 493.06	   31406
 493.33	   31645
 493.61	   31728
 493.89	   31805
 494.17	   31983
 494.44	   32032
 494.72	   32131
 495.00	   32227
 495.28	   32290
 495.56	   32559
 495.83	   32646
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