# Optical absorption spectrum of Tryptophan in water, 0.1 M phosphate buffer, pH 7 # # Data from PhotochemCAD 2.1a has been munged on 2 June 2017 by Scott Prahl to make # the information available to non-Windows users. Although I have tried to be as careful # as possible, I may have introduced some error; the cautious user is advised to compare # these results with the original sources at www.photochemcad.com: # # H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design # and research tool in photochemistry," Photochem. Photobiol., 68, 141-142, 1998. # J. M. Dixon, M. Taniguchi and J. S. Lindsey "PhotochemCAD 2. A refined program with accompanying # spectral databases for photochemical calculations", Photochem. Photobiol., 81, 212-213, 2005. # # The spectral absorption measurements were made by R.-C. A. Fuh in the summer of 1995 using a Cary 3. # The absorption values were collected using a spectral bandwidth of 1.0 nm, a signal averaging time of 0.133 sec, a data interval of 0.25 nm, and a scan rate of 112.5 nm/min. # # These measurements were scaled to make the molar extinction coefficient match # the value of 5579 cm-1/M at 278.0 nm. These values were # then interpolated to report extinction coefficients at regular 1nm intervals # # The reported molar extinction coefficient is from # Fasman, G. D., Editor (1976) Handbook of Biochemistry and Molecular Biology, 3rd Edition, Proteins, Volume I, pp. 183-203, CRC Press, Cleveland, Ohio. # ##Wavelength (nm) Molar Extinction (cm-1/M) 219.75 12488 220 12872 220.25 12428 220.5 12882 220.75 12456 221 12270 221.25 12611 221.5 12726 221.75 12756 222 12225 222.25 12711 222.5 12137 222.75 12408 223 12240 223.25 12596 223.5 12254 223.75 12415 224 12604 224.25 12258 224.5 12342 224.75 12280 225 12049 225.25 12689 225.5 12109 225.75 11786 226 12054 226.25 11938 226.5 11832 226.75 11708 227 11522 227.25 11158 227.5 10931 227.75 10576 228 10210 228.25 9771 228.5 9325 228.75 8840 229 8319 229.25 7811 229.5 7325 229.75 6861 230 6417 230.25 6049 230.5 5679 230.75 5338 231 4984 231.25 4709 231.5 4426 231.75 4181 232 3953 232.25 3752 232.5 3577 232.75 3412 233 3258 233.25 3116 233.5 3000 233.75 2881 234 2788 234.25 2684 234.5 2590 234.75 2497 235 2410 235.25 2334 235.5 2265 235.75 2203 236 2160 236.25 2117 236.5 2068 236.75 2028 237 1993 237.25 1962 237.5 1938 237.75 1919 238 1896 238.25 1881 238.5 1853 238.75 1831 239 1821 239.25 1808 239.5 1794 239.75 1773 240 1768 240.25 1753 240.5 1751 240.75 1747 241 1744 241.25 1751 241.5 1750 241.75 1742 242 1745 242.25 1745 242.5 1753 242.75 1741 243 1746 243.25 1759 243.5 1763 243.75 1774 244 1790 244.25 1789 244.5 1805 244.75 1817 245 1824 245.25 1832 245.5 1842 245.75 1865 246 1872 246.25 1893 246.5 1914 246.75 1936 247 1952 247.25 1971 247.5 1990 247.75 2007 248 2036 248.25 2055 248.5 2080 248.75 2094 249 2118 249.25 2151 249.5 2174 249.75 2208 250 2240 250.25 2261 250.5 2282 250.75 2320 251 2351 251.25 2379 251.5 2415 251.75 2443 252 2478 252.25 2513 252.5 2557 252.75 2589 253 2626 253.25 2663 253.5 2707 253.75 2745 254 2780 254.25 2814 254.5 2848 254.75 2861 255 2890 255.25 2925 255.5 2969 255.75 3003 256 3066 256.25 3113 256.5 3154 256.75 3181 257 3224 257.25 3287 257.5 3315 257.75 3353 258 3415 258.25 3447 258.5 3498 258.75 3529 259 3578 259.25 3627 259.5 3681 259.75 3721 260 3765 260.25 3808 260.5 3865 260.75 3913 261 3957 261.25 4003 261.5 4036 261.75 4090 262 4142 262.25 4204 262.5 4232 262.75 4286 263 4315 263.25 4368 263.5 4400 263.75 4438 264 4492 264.25 4518 264.5 4565 264.75 4617 265 4650 265.25 4689 265.5 4712 265.75 4743 266 4781 266.25 4825 266.5 4847 266.75 4895 267 4921 267.25 4931 267.5 4971 267.75 5008 268 5022 268.25 5058 268.5 5076 268.75 5104 269 5121 269.25 5170 269.5 5177 269.75 5213 270 5237 270.25 5256 270.5 5276 270.75 5287 271 5298 271.25 5321 271.5 5309 271.75 5342 272 5360 272.25 5332 272.5 5350 272.75 5345 273 5344 273.25 5333 273.5 5323 273.75 5340 274 5357 274.25 5337 274.5 5351 274.75 5373 275 5378 275.25 5413 275.5 5433 275.75 5456 276 5461 276.25 5492 276.5 5505 276.75 5506 277 5570 277.25 5552 277.5 5569 277.75 5554 278 5579 278.25 5567 278.5 5560 278.75 5571 279 5566 279.25 5573 279.5 5562 279.75 5563 280 5501 280.25 5518 280.5 5469 280.75 5459 281 5431 281.25 5390 281.5 5317 281.75 5253 282 5208 282.25 5157 282.5 5075 282.75 5009 283 4979 283.25 4880 283.5 4807 283.75 4750 284 4703 284.25 4653 284.5 4601 284.75 4572 285 4544 285.25 4522 285.5 4508 285.75 4514 286 4523 286.25 4558 286.5 4579 286.75 4581 287 4594 287.25 4615 287.5 4627 287.75 4598 288 4584 288.25 4513 288.5 4456 288.75 4390 289 4289 289.25 4170 289.5 4045 289.75 3902 290 3771 290.25 3601 290.5 3476 290.75 3308 291 3159 291.25 3021 291.5 2878 291.75 2746 292 2609 292.25 2482 292.5 2359 292.75 2244 293 2130 293.25 2025 293.5 1926 293.75 1837 294 1748 294.25 1663 294.5 1614 294.75 1580 295 1532 295.25 1449 295.5 1384 295.75 1314 296 1237 296.25 1178 296.5 1114 296.75 1054 297 1001 297.25 947 297.5 901 297.75 860 298 816 298.25 763 298.5 723 298.75 682 299 646 299.25 606 299.5 568 299.75 547 300 507 300.25 485 300.5 453 300.75 438 301 406 301.25 378 301.5 360 301.75 331 302 316 302.25 295 302.5 273 302.75 265 303 248 303.25 234 303.5 219 303.75 197 304 195 304.25 176 304.5 166 304.75 151 305 150 305.25 140 305.5 135 305.75 128 306 113 306.25 112 306.5 103 306.75 96 307 91 307.25 89 307.5 76 307.75 73 308 67 308.25 70 308.5 66 308.75 61 309 63 309.25 43 309.5 51 309.75 72 310 61 310.25 81 310.5 70 310.75 60 311 58 311.25 45 311.5 20 311.75 46 312 26 312.25 40 312.5 37 312.75 40 313 0 313.25 19 313.5 0 313.75 21 314 55 314.25 16 314.5 6 314.75 32 315 8 315.25 33 315.5 6 315.75 3 316 8 316.25 9 316.5 13 316.75 15 317 14 317.25 15 317.5 8 317.75 0 318 32 318.25 16 318.5 14 318.75 14 319 18 319.25 45 319.5 14 319.75 2 320 -3 320.25 1 320.5 19 320.75 -6 321 0 321.25 16 321.5 4 321.75 17 322 -3 322.25 -17 322.5 -5 322.75 -1 323 3 323.25 11 323.5 12 323.75 2 324 -7 324.25 4 324.5 -6 324.75 17 325 -19 325.25 14 325.5 -5 325.75 -11 326 -1 326.25 5 326.5 21 326.75 1 327 12 327.25 21 327.5 12 327.75 -14 328 6 328.25 4 328.5 34 328.75 12 329 9 329.25 6 329.5 -13 329.75 -1 330 -3 330.25 -1 330.5 -10 330.75 2 331 -11 331.25 20 331.5 -5 331.75 4 332 -8 332.25 0 332.5 7 332.75 6 333 -5 333.25 10 333.5 -10 333.75 -2 334 12 334.25 -7 334.5 13 334.75 10 335 -3 335.25 2 335.5 -1 335.75 16 336 -6 336.25 -9 336.5 -5 336.75 2 337 6 337.25 3 337.5 -6 337.75 7 338 6 338.25 -5 338.5 -1 338.75 -10 339 -4 339.25 -5 339.5 -6 339.75 -9 340 3 340.25 4 340.5 -3 340.75 -9 341 -3 341.25 3 341.5 0 341.75 -12 342 -8 342.25 -15 342.5 -2 342.75 6 343 2 343.25 -1 343.5 -3 343.75 -2 344 -19 344.25 4 344.5 11 344.75 2 345 2 345.25 4 345.5 1 345.75 -2 346 -11 346.25 0 346.5 2 346.75 -2 347 10 347.25 -5 347.5 2 347.75 -5 348 1 348.25 0 348.5 -4 348.75 0 349 -6 349.25 -13 349.5 -3 349.75 6 350 6 350.25 2 350.5 -11 350.75 -6 351 -3 351.25 8 351.5 5 351.75 3 352 0 352.25 -14 352.5 4 352.75 0 353 5 353.25 2 353.5 4 353.75 -1 354 6 354.25 -1 354.5 1 354.75 -10 355 -6 355.25 -6 355.5 1 355.75 -5 356 -1 356.25 5 356.5 -6 356.75 -5 357 -3 357.25 4 357.5 -5 357.75 -7 358 0 358.25 1 358.5 2 358.75 -2 359 8 359.25 7 359.5 3 359.75 3 360 0 360.25 2 360.5 5 360.75 6 361 5 361.25 6 361.5 1 361.75 -8 362 -10 362.25 -7 362.5 -2 362.75 0 363 2 363.25 -7 363.5 -6 363.75 -5 364 2 364.25 -8 364.5 -3 364.75 7 365 3 365.25 -3 365.5 -1 365.75 -7 366 3 366.25 1 366.5 -5 366.75 -7 367 6 367.25 0 367.5 0 367.75 -2 368 9 368.25 -1 368.5 -3 368.75 -12 369 -4 369.25 7 369.5 -12 369.75 -4 370 -8 370.25 -1 370.5 1 370.75 5 371 7 371.25 15 371.5 -6 371.75 -6 372 -3 372.25 -5 372.5 7 372.75 -7 373 -5 373.25 -3 373.5 -6 373.75 0 374 -2 374.25 8 374.5 -5 374.75 -8 375 4 375.25 2 375.5 -15 375.75 -2 376 0 376.25 3 376.5 -1 376.75 -2 377 -2 377.25 -3 377.5 -7 377.75 -8 378 8 378.25 -1 378.5 -1 378.75 -4 379 -1 379.25 0 379.5 -7 379.75 0 380 -8 380.25 -2 380.5 -14 380.75 -2 381 -3 381.25 -4 381.5 -7 381.75 -1 382 -3 382.25 6 382.5 -5 382.75 0 383 4 383.25 -8 383.5 -6 383.75 -7 384 -13 384.25 -2 384.5 -16 384.75 0 385 1 385.25 3 385.5 3 385.75 -1 386 -7 386.25 -4 386.5 2 386.75 -5 387 -5 387.25 5 387.5 -3 387.75 5 388 3 388.25 -5 388.5 -9 388.75 -8 389 -2 389.25 0 389.5 -7 389.75 -3 390 -2 390.25 -9 390.5 3 390.75 -3 391 -11 391.25 -7 391.5 -3 391.75 6 392 1 392.25 -5 392.5 -7 392.75 -8 393 -1 393.25 -6 393.5 -6 393.75 1 394 6 394.25 -3 394.5 -5 394.75 -5 395 -5 395.25 -6 395.5 -13 395.75 -8 396 -2 396.25 0 396.5 -5 396.75 -5 397 -8 397.25 -9 397.5 -11 397.75 2 398 -6 398.25 -2 398.5 0 398.75 -9 399 -1 399.25 -3 399.5 -8 399.75 -12 400 -1 400.25 -4 400.5 -10 400.75 3 401 -12 401.25 -6 401.5 -3 401.75 -1 402 -6 402.25 -12 402.5 -2 402.75 -9 403 -5 403.25 -6 403.5 -5 403.75 -3 404 -8 404.25 -9 404.5 -8 404.75 -3 405 -14 405.25 -2 405.5 -2 405.75 -4 406 -8 406.25 0 406.5 -3 406.75 -8 407 2 407.25 -10 407.5 -15 407.75 -12 408 -1 408.25 -6 408.5 -7 408.75 -6 409 -12 409.25 -2 409.5 -6 409.75 -6 410 -7 410.25 2 410.5 -9 410.75 -10 411 -2 411.25 -11 411.5 -12 411.75 -2 412 -9 412.25 -6 412.5 -15 412.75 -9 413 -7 413.25 -8 413.5 -12 413.75 -12 414 -14 414.25 -8 414.5 -11 414.75 -4 415 -4 415.25 -9 415.5 -5 415.75 -9 416 -5 416.25 -6 416.5 2 416.75 -9 417 -8 417.25 -11 417.5 -6 417.75 -8 418 -4 418.25 -10 418.5 -3 418.75 -7 419 -3 419.25 -13 419.5 -8 419.75 -10 420 -5 420.25 -4 420.5 -4 420.75 -2 421 -12 421.25 -3 421.5 -6 421.75 -7 422 -7 422.25 -9 422.5 -10 422.75 -1 423 -15 423.25 -12 423.5 -7 423.75 -9 424 -7 424.25 0 424.5 -12 424.75 -4 425 -11 425.25 -5 425.5 -5 425.75 -11 426 -8 426.25 -13 426.5 -9 426.75 -4 427 -7 427.25 -3 427.5 -9 427.75 -11 428 -14 428.25 -9 428.5 -9 428.75 -6 429 -10 429.25 -7 429.5 -1 429.75 -8 430 -10 430.25 -9 430.5 -14 430.75 -10 431 -9 431.25 -6 431.5 -10 431.75 -6 432 -10 432.25 -5 432.5 -15 432.75 -10 433 -3 433.25 -5 433.5 -9 433.75 -10 434 -3 434.25 -2 434.5 -4 434.75 -7 435 -8 435.25 -4 435.5 -2 435.75 -13 436 -5 436.25 -11 436.5 -11 436.75 -11 437 -10 437.25 -9 437.5 -9 437.75 -10 438 -21 438.25 -11 438.5 -10 438.75 -7 439 -8 439.25 -7 439.5 -8 439.75 -6 440 -5 440.25 -4 440.5 -7 440.75 -12 441 -9 441.25 -8 441.5 -8 441.75 -10 442 -13 442.25 -17 442.5 -4 442.75 -6 443 -1 443.25 -12 443.5 -12 443.75 -8 444 -3 444.25 -12 444.5 -5 444.75 -8 445 -9 445.25 -5 445.5 -11 445.75 -13 446 -13 446.25 -7 446.5 -19 446.75 -16 447 -6 447.25 -9 447.5 -8 447.75 -12 448 -23 448.25 -8 448.5 -6 448.75 -7 449 -12 449.25 -14 449.5 -10 449.75 -10 450 -12 450.25 -5 450.5 -10 450.75 -9 451 -8 451.25 -9 451.5 -13 451.75 -4 452 -6 452.25 -6 452.5 -12 452.75 -12 453 -8 453.25 -12 453.5 -15 453.75 -12 454 -8 454.25 -5 454.5 -12 454.75 -17 455 -10 455.25 -12 455.5 -11 455.75 -10 456 -6 456.25 -15 456.5 -3 456.75 -14 457 -14 457.25 -11 457.5 -11 457.75 -9 458 -8 458.25 -14 458.5 -8 458.75 -15 459 -11 459.25 -15 459.5 -18 459.75 -12 460 -11 460.25 -13 460.5 -7 460.75 -10 461 -2 461.25 -12 461.5 -7 461.75 -20 462 -13 462.25 -5 462.5 -8 462.75 -17 463 -15 463.25 -2 463.5 -16 463.75 -9 464 -13 464.25 -18 464.5 -12 464.75 -13 465 -17 465.25 -15 465.5 -7 465.75 -11 466 -11 466.25 -12 466.5 -13 466.75 -11 467 -8 467.25 -17 467.5 -20 467.75 -10 468 -13 468.25 -12 468.5 -8 468.75 -12 469 -13 469.25 -12 469.5 -7 469.75 -7 470 -11 470.25 -14 470.5 -11 470.75 -8 471 -13 471.25 -17 471.5 -4 471.75 -6 472 -14 472.25 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498.25 -11 498.5 -16 498.75 -10 499 -14 499.25 -17 499.5 -17 499.75 -19 500 -5 500.25 -16 500.5 -10 500.75 -11 501 -7 501.25 -5 501.5 -14 501.75 -9 502 -18 502.25 -14 502.5 -13 502.75 -10 503 -11 503.25 -21 503.5 -22 503.75 -23 504 -13 504.25 -10 504.5 -19 504.75 -17 505 -14 505.25 -13 505.5 -10 505.75 -18 506 -20 506.25 -16 506.5 -18 506.75 -17 507 -14 507.25 -21 507.5 -15 507.75 -15 508 -14 508.25 -7 508.5 -15 508.75 -20 509 -11 509.25 -16 509.5 -16 509.75 -12 510 -10 510.25 -19 510.5 -12 510.75 -17 511 -19 511.25 -15 511.5 -19 511.75 -19 512 -7 512.25 -15 512.5 -13 512.75 -8 513 -18 513.25 -25 513.5 -14 513.75 -18 514 -22 514.25 -17 514.5 -16 514.75 -12 515 -23 515.25 -22 515.5 -17 515.75 -16 516 -20 516.25 -16 516.5 -19 516.75 -20 517 -13 517.25 -17 517.5 -20 517.75 -13 518 -11 518.25 -21 518.5 -12 518.75 -12 519 -11 519.25 -18 519.5 -20 519.75 -12 520 -18 520.25 -8 520.5 -12 520.75 -17 521 -16 521.25 -12 521.5 -21 521.75 -20 522 -16 522.25 -16 522.5 -23 522.75 -21 523 -14 523.25 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548.5 -20 548.75 -19 549 -19 549.25 -15 549.5 -19 549.75 -24 550 -22 550.25 -25 550.5 -21 550.75 -24 551 -15 551.25 -16 551.5 -23 551.75 -17 552 -18 552.25 -14 552.5 -27 552.75 -18 553 -23 553.25 -25 553.5 -18 553.75 -20 554 -21 554.25 -10 554.5 -21 554.75 -19 555 -18 555.25 -22 555.5 -16 555.75 -15 556 -27 556.25 -21 556.5 -25 556.75 -15 557 -22 557.25 -23 557.5 -13 557.75 -17 558 -24 558.25 -30 558.5 -16 558.75 -24 559 -27 559.25 -21 559.5 -23 559.75 -22 560 -27 560.25 -19 560.5 -24 560.75 -19 561 -15 561.25 -18 561.5 -29 561.75 -15 562 -24 562.25 -15 562.5 -26 562.75 -21 563 -19 563.25 -19 563.5 -27 563.75 -22 564 -10 564.25 -15 564.5 -15 564.75 -25 565 -23 565.25 -15 565.5 -23 565.75 -21 566 -26 566.25 -17 566.5 -18 566.75 -21 567 -21 567.25 -21 567.5 -19 567.75 -15 568 -15 568.25 -20 568.5 -16 568.75 -21 569 -15 569.25 -18 569.5 -16 569.75 -8 570 -12 570.25 -12 570.5 -13 570.75 -14 571 -10 571.25 -12 571.5 -15 571.75 -11 572 -9 572.25 -12 572.5 -15 572.75 -21 573 -16 573.25 -16 573.5 -4 573.75 -25 574 -15 574.25 -13 574.5 -14 574.75 -10 575 -10 575.25 -21 575.5 -14 575.75 -13 576 -18 576.25 -11 576.5 -14 576.75 -12 577 -14 577.25 -19 577.5 -12 577.75 -16 578 -8 578.25 -21 578.5 -15 578.75 -6 579 -14 579.25 -23 579.5 -13 579.75 -13 580 -17 580.25 -10 580.5 -15 580.75 -12 581 -8 581.25 -19 581.5 -18 581.75 -17 582 -18 582.25 -14 582.5 -21 582.75 -18 583 -20 583.25 -9 583.5 -16 583.75 -17 584 -10 584.25 -7 584.5 -14 584.75 -19 585 -16 585.25 -5 585.5 -14 585.75 -16 586 -17 586.25 -10 586.5 -18 586.75 -19 587 -12 587.25 -12 587.5 -9 587.75 -10 588 -10 588.25 -12 588.5 -12 588.75 -14 589 -24 589.25 -11 589.5 -16 589.75 -14 590 -16 590.25 -20 590.5 -14 590.75 -14 591 -15 591.25 -12 591.5 -15 591.75 -15 592 -13 592.25 -7 592.5 -17 592.75 -13 593 -10 593.25 -10 593.5 -24 593.75 -23 594 -12 594.25 -18 594.5 -12 594.75 -18 595 -8 595.25 -11 595.5 -15 595.75 -15 596 -14 596.25 -10 596.5 -17 596.75 -9 597 -24 597.25 -8 597.5 -7 597.75 -22 598 -12 598.25 -15 598.5 -15 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