Indocarbocyanine (C3)dye

Absorption

This is a graph of the molar extinction coefficient of Indocarbocyanine (C3)dye dissolved in methanol. It was measured by R. A. Fuh on 6/21/95 [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. Indocarbocyanine (C3)dye has a molar extinction coefficient of 133,000 M^-1cm^-1 at 544.25 nm [P. J. Sims, A. S. Waggoner, C.-H. Wang, and J. F. Hoffman, "Studies on the mechanism by which cyanine dyes measure membrane potential in red blood cells and phosphatidylcholine vesicles," Biochem., 13, 3315-3330, 1974].

The literature absorption data were obtained in ethanol. Other fluorescence yields include 0.057 in n-butyronitrile [J. S. Lindsey, P. A. Brown, and D. A. Siesel, "Visible light harvesting in covalently-linked porphyrin-cyanine dyes," Tetrahedron, 45, 4845-4866, 1989], and 0.08 in 95% ethanol for an n-hexyl-substituted dye [F. Grieser, M. Lay, and P. J. Thistlethwaite, "Excited-state torsional relaxation in 1,1'-dihexyl-3,3,3',3'-tetramethylindocarbocyanine iodide: Application to the probing of micelle structure.," J. Phys. Chem., 89, 2065-2070, 1985].

Original Data | Extinction Data

Fluorescence

This is the fluorescence emission spectrum of Indocarbocyanine (C3)dye dissolved in methanol. The spectrum was taken by R. A. Fuh on 6/21/95 using an excitation wavelength of 500 nm [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. The quantum yield is 0.07 [A. Waggoner, R. DeBiasio, P. Conrad, G. R. Bright, L. Ernst, K. Ryan, M. Nederlof, and D. Taylor, "Multiple spectral parameter imaging," Methods in Cell Biology, 30, 449-478, 1989].

Original Data