Indodicarbocyanine (C5)dye |
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| The chemical structure of Indodicarbocyanine (C5)dye is shown at the right. The absorption and fluorescence emission spectra are shown below. The structure, absorption, and fluorescence data were all obtained from the PhotochemCAD package by Jonathan Lindsey. This excellent program allows rapid comparison of spectra and enables one to do a variety of photochemically relevant calculations. |
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Absorption
This is a graph of the molar extinction coefficient of Indodicarbocyanine (C5)dye dissolved in methanol. It was measured by R. A. Fuh on 6/21/95 [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. Indodicarbocyanine (C5)dye has a molar extinction coefficient of 200,000 M-1cm-1 at 646 nm [A. Waggoner, R. DeBiasio, P. Conrad, G. R. Bright, L. Ernst, K. Ryan, M. Nederlof, and D. Taylor, "Multiple spectral parameter imaging," Methods in Cell Biology, 30, 449-478, 1989]. The literature absorption data were obtained in ethanol. |
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Fluorescence
This is the fluorescence emission spectrum of Indodicarbocyanine (C5)dye dissolved in methanol. The spectrum was taken by R. A. Fuh on 6/21/95 using an excitation wavelength of 590 nm [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. The quantum yield is 0.40 [P. J. Sims, A. S. Waggoner, C.-H. Wang, and J. F. Hoffman, "Studies on the mechanism by which cyanine dyes measure membrane potential in red blood cells and phosphatidylcholine vesicles," Biochem., 13, 3315-3330, 1974]. |
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