Acridine orange |
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| The chemical structure of Acridine orange is shown at the right. The absorption and fluorescence emission spectra are shown below. The structure, absorption, and fluorescence data were all obtained from the PhotochemCAD package by Jonathan Lindsey. This excellent program allows rapid comparison of spectra and enables one to do a variety of photochemically relevant calculations. |
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Absorption
This is a graph of the molar extinction coefficient of Acridine orange dissolved in basic ethanol. It was measured by R. A. Fuh on summer, 95 [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. Acridine orange has a molar extinction coefficient of 27,000 M-1cm-1 at 430.75 nm [J. Ferguson and A. W. H. Mau, "Absorption studies of acid-base equilibria of dye solutions," Chem. Phys. Lett., 17, 543-546, 1972].
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Fluorescence
This is the fluorescence emission spectrum of Acridine orange dissolved in basic ethanol. The spectrum was taken by R. A. Fuh on summer, 95 using an excitation wavelength of 400 nm [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. The quantum yield is 0.20 [B. Soep, A. Kellmann, M. Martin, and L. Lindqvist, "Study of triplet quantum yields using a tunable dye laser.," Chem. Phys. Lett., 13, 241-244, 1972]. |
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