Acridine yellow |
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| The chemical structure of Acridine yellow is shown at the right. The absorption and fluorescence emission spectra are shown below. The structure, absorption, and fluorescence data were all obtained from the PhotochemCAD package by Jonathan Lindsey. This excellent program allows rapid comparison of spectra and enables one to do a variety of photochemically relevant calculations. |
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Absorption
This is a graph of the molar extinction coefficient of Acridine yellow dissolved in ethanol. It was measured by R. A. Fuh on summer, 95 [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. Acridine yellow has a molar extinction coefficient of 39,400 M-1cm-1 at 460.75 nm [I. B. Berlman, Handbook of Fluorescence Spectra of Aromatic Molecules, Academic Press, 1971].
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Fluorescence
This is the fluorescence emission spectrum of Acridine yellow dissolved in ethanol. The spectrum was taken by R. A. Fuh on summer, 95 using an excitation wavelength of 420 nm [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. The quantum yield is 0.47 [J. I. I. I. Olmsted, "Calorimetric determinations of absolute fluorescence quantum yields.," J. Phys. Chem., 83, 2581-2584, 1979]. |
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