Auramine O |
|
| The chemical structure of Auramine O is shown at the right. The absorption and fluorescence emission spectra are shown below. The structure, absorption, and fluorescence data were all obtained from the PhotochemCAD package by Jonathan Lindsey. This excellent program allows rapid comparison of spectra and enables one to do a variety of photochemically relevant calculations. |
|
Absorption
This is a graph of the molar extinction coefficient of Auramine O dissolved in water. It was measured by R. A. Fuh on 6/12/95 [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. Auramine O has a molar extinction coefficient of 25,300 M-1cm-1 at 431.25 nm [W. Muller and F. Gautier, "Interactions of heteroaromatic compounds with nucleic acids. A.T-specific non-intercalating DNA ligands.," Eur. J. Biochem., 54, 385-394, 1975]. The fluorescence yield varies markedly with solvent viscosity, ranging from 0.0016 in methanol to 0.019 in n-decanol [P. Gautam and A. Harriman, "Internal rotation in Auramine O," J. Chem. Soc. Faraday Trans., 90, 697-701, 1994]. |
|
Fluorescence
This is the fluorescence emission spectrum of Auramine O dissolved in glycerol. The spectrum was taken by R. A. Fuh on 6/12/95 using an excitation wavelength of 400 nm [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. The quantum yield is 0.03 [L. A. Brey, G. B. Schuster, and H. G. Drickamer, "High pressure studies of the effect of viscosity on fluorescence efficiency in crystal violet and auramine O," J. Chem. Phys., 67, 2648-2650, 1977]. |
|