Chlorophyll a, methanol |
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| The chemical structure of Chlorophyll a, methanol is shown at the right. The absorption and fluorescence emission spectra are shown below. The structure, absorption, and fluorescence data were all obtained from the PhotochemCAD package by Jonathan Lindsey. This excellent program allows rapid comparison of spectra and enables one to do a variety of photochemically relevant calculations. |
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Absorption
This is a graph of the molar extinction coefficient of Chlorophyll a dissolved in methanol. It was measured by Junzhong Li on 12/11/97 [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. Chlorophyll a, methanol has a molar extinction coefficient of 111,700 M-1cm-1 at 417.8 nm [H. H. Strain, M. R. Thomas, and J. J. Katz, "Spectral absorption properties of ordinary and fully deuteriated chlorophylls a and b," Biochim. Biophys. Acta, 75, 306-311, 1963]. The absorption spectrum of chlorophyll a has been measured in 40 solvents [G. R. Seely and R. G. Jensen, "Effect of solvent on the spectrum of chlorophyll," Spectrochim. Acta, 21, 1835-1845, 1965]. For fluorescence studies of diverse photosynthetic pigments, see [G. R. Seely and J. S. Connolly, "Fluorescence of photosynthetic pigments in vitro," in Light emission by plants and bacteria, (J. Govindjee and D. C. Fork, eds.), 99-133, 1986]. |
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Fluorescence
This is the fluorescence emission spectrum of Chlorophyll a dissolved in methanol. The spectrum was taken by Junzhong Li on 12/11/97 using an excitation wavelength of 417 nm [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. The quantum yield is 0.32 [G. Weber and F. W. J. Teale, "Determination of the absolute quantum yield of fluorescent solutions," Trans. Far. Trans., 53, 646-655, 1957]. |
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