Chlorophyll a, diethyl ether |
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| The chemical structure of Chlorophyll a, diethyl ether is shown at the right. The absorption and fluorescence emission spectra are shown below. The structure, absorption, and fluorescence data were all obtained from the PhotochemCAD package by Jonathan Lindsey. This excellent program allows rapid comparison of spectra and enables one to do a variety of photochemically relevant calculations. |
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Absorption
This is a graph of the molar extinction coefficient of Chlorophyll a dissolved in diethyl ether. It was measured by Junzhong Li on 12/11/97 [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. Chlorophyll a, diethyl ether has a molar extinction coefficient of 111,700 M-1cm-1 at 427.75 nm [H. H. Strain, M. R. Thomas, and J. J. Katz, "Spectral absorption properties of ordinary and fully deuteriated chlorophylls a and b," Biochim. Biophys. Acta, 75, 306-311, 1963]. For fluorescence studies of diverse photosynthetic pigments, see [G. R. Seely and J. S. Connolly, "Fluorescence of photosynthetic pigments in vitro," in Light emission by plants and bacteria, (J. Govindjee and D. C. Fork, eds.), 99-133, 1986]. |
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Fluorescence
This is the fluorescence emission spectrum of Chlorophyll a dissolved in diethyl ether. The spectrum was taken by Junzhong Li on 12/11/97 using an excitation wavelength of 614 nm [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. The quantum yield is 0.32 [G. Weber and F. W. J. Teale, "Determination of the absolute quantum yield of fluorescent solutions," Trans. Far. Trans., 53, 646-655, 1957]. |
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