Crystal violet, glycerol |
|
| The chemical structure of Crystal violet, glycerol is shown at the right. The absorption and fluorescence emission spectra are shown below. The structure, absorption, and fluorescence data were all obtained from the PhotochemCAD package by Jonathan Lindsey. This excellent program allows rapid comparison of spectra and enables one to do a variety of photochemically relevant calculations. |
|
Absorption
This is a graph of the molar extinction coefficient of Crystal violet dissolved in glycerol. It was measured by R. A. Fuh on summer, 95 [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. Crystal violet, glycerol has a molar extinction coefficient of 112,000 M-1cm-1 at 590.25 nm [H. Zollinger, Color Chemistry: Syntheses Properties and Applications of Organic Dyes and Pigments, VCH Verlagsgesellschaft mbH, 1987].
|
|
Fluorescence
This is the fluorescence emission spectrum of Crystal violet dissolved in glycerol. The spectrum was taken by R. A. Fuh on summer, 95 using an excitation wavelength of 550 nm [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. The quantum yield is 0.019 [L. A. Brey, G. B. Schuster, and H. G. Drickamer, "High pressure studies of the effect of viscosity on fluorescence efficiency in crystal violet and auramine O," J. Chem. Phys., 67, 2648-2650, 1977]. |
|