Dansyl glycine, dioxane |
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| The chemical structure of Dansyl glycine, dioxane is shown at the right. The absorption and fluorescence emission spectra are shown below. The structure, absorption, and fluorescence data were all obtained from the PhotochemCAD package by Jonathan Lindsey. This excellent program allows rapid comparison of spectra and enables one to do a variety of photochemically relevant calculations. |
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Absorption
This is a graph of the molar extinction coefficient of Dansyl glycine dissolved in dioxane. It was measured by R. A. Fuh on summer, 97 [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. Dansyl glycine, dioxane has a molar extinction coefficient of 4,300 M-1cm-1 at 338.5 nm [R. F. Chen, "Dansyl labeled proteins: Determination of extinction coefficient and number of bound residues with radioactive dansyl chloride," Anal. Biochem., 25, 412-416, 1968]. The fluorescence yield ranges from 0.07 in water to 0.66 in dioxane [J. Bramhall, "Phospholipid packing asymmetry in curved membranes detected by fluorescence spectroscopy," Biochemistry, 25, 3479-3486, 1986]. The fluorescence yield also has been reported to be 0.065 in water [R. F. Chen, "Fluorescence of dansyl amino acids in organic solvents and protein solutions," Arch. Biochem. Biophys., 120, 609-620, 1967]. |
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Fluorescence
This is the fluorescence emission spectrum of Dansyl glycine dissolved in dioxane. The spectrum was taken by R. A. Fuh on summer, 97 using an excitation wavelength of 300 nm [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. The quantum yield is 0.66 [J. Bramhall, "Phospholipid packing asymmetry in curved membranes detected by fluorescence spectroscopy," Biochemistry, 25, 3479-3486, 1986]. |
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