DAPI |
|
| The chemical structure of 4',6-Diamidino-2-phenylindole (DAPI), dimethylsulfoxide is shown at the right. The absorption and fluorescence emission spectra are shown below. The structure, absorption, and fluorescence data were all obtained from the PhotochemCAD package by Jonathan Lindsey. This excellent program allows rapid comparison of spectra and enables one to do a variety of photochemically relevant calculations. |
|
Absorption
This is a graph of the molar extinction coefficient of 4',6-Diamidino-2-phenylindole (DAPI) dissolved in dimethylsulfoxide. It was measured by R. A. Fuh on summer, 97 [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. 4',6-Diamidino-2-phenylindole (DAPI), dimethylsulfoxide has a molar extinction coefficient of 27,000 M-1cm-1 at 353 nm [R. P. Haugland, Molecular Probes. Handbook of Fluorescent Probes and Research Chemicals, 1992].
|
|
Fluorescence
This is the fluorescence emission spectrum of 4',6-Diamidino-2-phenylindole (DAPI) dissolved in dimethylsulfoxide. The spectrum was taken by R. A. Fuh on summer, 97 using an excitation wavelength of 310 nm [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. The quantum yield is 0.58 [T. Hard, P. Fan, and D. R. Kearns, "A fluorescence study of the binding of Hoechst 33258 and DAPI to halogenated DNAs," Photochem. Photobiol., 51, 77-86, 1990]. |
|