Fluorescein, basic ethanol |
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| The chemical structure of Fluorescein, basic ethanol is shown at the right. The absorption and fluorescence emission spectra are shown below. The structure, absorption, and fluorescence data were all obtained from the PhotochemCAD package by Jonathan Lindsey. This excellent program allows rapid comparison of spectra and enables one to do a variety of photochemically relevant calculations. |
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Absorption
This is a graph of the molar extinction coefficient of Fluorescein dissolved in basic ethanol. It was measured by R. A. Fuh on 6/20/95 [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. Fluorescein, basic ethanol has a molar extinction coefficient of 92,300 M-1cm-1 at 500.25 nm [P. G. Seybold, M. Gouterman, and J. Callis, "Calorimetric, photometric and lifetime determinations of fluorescence yields of fluorescein dyes," Photochem. Photobiol., 9, 229-242, 1969].
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Fluorescence
This is the fluorescence emission spectrum of Fluorescein dissolved in basic ethanol. The spectrum was taken by R. A. Fuh on 6/20/95 using an excitation wavelength of 470 nm [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. The quantum yield is 0.97 [P. G. Seybold, M. Gouterman, and J. Callis, "Calorimetric, photometric and lifetime determinations of fluorescence yields of fluorescein dyes," Photochem. Photobiol., 9, 229-242, 1969]. |
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