Hoechst 33258, H2O |
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| The chemical structure of Hoechst 33258, H2O is shown at the right. The absorption and fluorescence emission spectra are shown below. The structure, absorption, and fluorescence data were all obtained from the PhotochemCAD package by Jonathan Lindsey. This excellent program allows rapid comparison of spectra and enables one to do a variety of photochemically relevant calculations. |
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Absorption
This is a graph of the molar extinction coefficient of Hoechst 33258, H2O dissolved in water. It was measured by R. A. Fuh on summer, 95 [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. Hoechst 33258, H2O has a molar extinction coefficient of 46,000 M-1cm-1 at 345.5 nm [R. P. Haugland, Molecular Probes. Handbook of Fluorescent Probes and Research Chemicals, 1992]. The fluorescence yield of Hoechst 33258 increases dramatically in the presence of DNA [B. Weisblum and E. Haenssler, "Fluorometric properties of the bibenzimidazole derivative Hoechst 33258, a fluorescent probe specific for AT concentration in chromosomal DNA," Chromosoma (Berl.), 46, 255-260, 1974] or in the solvent DMF. |
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Fluorescence
This is the fluorescence emission spectrum of Hoechst 33258, H2O dissolved in water. The spectrum was taken by R. A. Fuh on 6/24/97 using an excitation wavelength of 350 nm [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. The quantum yield is 0.034 [T. Hard, P. Fan, and D. R. Kearns, "A fluorescence study of the binding of Hoechst 33258 and DAPI to halogenated DNAs," Photochem. Photobiol., 51, 77-86, 1990]. |
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