Merocyanine 540 |
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| The chemical structure of Merocyanine 540 is shown at the right. The absorption and fluorescence emission spectra are shown below. The structure, absorption, and fluorescence data were all obtained from the PhotochemCAD package by Jonathan Lindsey. This excellent program allows rapid comparison of spectra and enables one to do a variety of photochemically relevant calculations. |
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Absorption
This is a graph of the molar extinction coefficient of Merocyanine 540 dissolved in ethanol. It was measured by R. A. Fuh on summer, 95 [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. Merocyanine 540 has a molar extinction coefficient of 138,000 M-1cm-1 at 560.25 nm [N. S. Dixit and R. A. Mackay, "Absoption and emission characteristics of Merocyanine 540 in microemulsions," J. Am. Chem. Soc., 105, 2928-2929, 1983]. The fluorescence yield has also been reported to be 0.29 [P. F. Aramendia, M. Krieg, C. Nitsch, E. Bittersmann, and S. E. Braslavsky, "The photophysics of merocyanine 540. A comparative study in ethanol and in liposomes.," Photochem. Photobiol., 48, 187-194, 1988] and 0.16 in ethanol [A. C. Benniston, A. Harriman, and K. S. Gulliya, "Photophysical properties of merocyanine 540 derivatives," J. Chem. Soc. Faraday Trans., 90, 953-961, 1994]. |
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Fluorescence
This is the fluorescence emission spectrum of Merocyanine 540 dissolved in ethanol. The spectrum was taken by R. A. Fuh on summer, 95 using an excitation wavelength of 530 nm [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. The quantum yield is 0.39 [M. Hoebeke, J. Piette, and A. vanĘde Vorst, "Viscosity-dependent isomerization and fluorescence yields of merocyanine 540.," J. Photochem. Photobiol., 4, 273-282, 1990]. |
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