Nile Blue, methanol |
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| The chemical structure of Nile Blue, methanol is shown at the right. The absorption and fluorescence emission spectra are shown below. The structure, absorption, and fluorescence data were all obtained from the PhotochemCAD package by Jonathan Lindsey. This excellent program allows rapid comparison of spectra and enables one to do a variety of photochemically relevant calculations. |
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Absorption
This is a graph of the molar extinction coefficient of Nile Blue dissolved in methanol. It was measured by R. A. Fuh on 6/14/95 [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. Nile Blue, methanol has a molar extinction coefficient of 76,800 M-1cm-1 at 626.75 nm [R. R. Birge, "Kodak Laser Dyes," 1987]. The fluorescence yield is 0.26 in ethylene glycol at 24 C, and 0.11 in ethanol at 61 C [R. Sens and K. H. Drexhage, "Fluorescence quantum yield of oxazine and carbazine laser dyes.," J. Luminesc., 24, 709-712, 1981]. |
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Fluorescence
This is the fluorescence emission spectrum of Nile Blue dissolved in methanol. The spectrum was taken by R. A. Fuh on 6/14/95 using an excitation wavelength of 540 nm [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. The quantum yield is 0.27 [R. Sens and K. H. Drexhage, "Fluorescence quantum yield of oxazine and carbazine laser dyes.," J. Luminesc., 24, 709-712, 1981]. |
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