Nile Red |
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| The chemical structure of Nile Red is shown at the right. The absorption and fluorescence emission spectra are shown below. The structure, absorption, and fluorescence data were all obtained from the PhotochemCAD package by Jonathan Lindsey. This excellent program allows rapid comparison of spectra and enables one to do a variety of photochemically relevant calculations. |
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Absorption
This is a graph of the molar extinction coefficient of Nile Red dissolved in Dioxane. It was measured by R. A. Fuh on 6/16/95 [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. Nile Red has a molar extinction coefficient of 38,000 M-1cm-1 at 519.4 nm [M. M. Davis and H. B. Hetzer, "Titrimetric and equilibrium studies using indicators related to Nile Blue A," Anal. Chem., 38, 451-461, 1966]. The absorption spectrum is solvent dependent [M. M. Davis and H. B. Hetzer, "Titrimetric and equilibrium studies using indicators related to Nile Blue A," Anal. Chem., 38, 451-461, 1966], as is the emission wavelength [M. R. V. Sahyun, "Total luminescence spectroscopy in a reverse micellar system.," J. Phys. Chem., 92, 6028-6032, 1988]. |
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Fluorescence
This is the fluorescence emission spectrum of Nile Red dissolved in Dioxane. The spectrum was taken by R. A. Fuh on 6/14/95 using an excitation wavelength of 460 nm [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. The quantum yield is 0.7 [D. L. Sackett and J. Wolff, "Nile red as a polarity-sensitive fluorescent probe of hydrophobic protein surfaces.," Anal. Biochem., 167, 228-234, 1987]. |
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