Proflavin, pH 7 |
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| The chemical structure of Proflavin, pH 7 is shown at the right. The absorption and fluorescence emission spectra are shown below. The structure, absorption, and fluorescence data were all obtained from the PhotochemCAD package by Jonathan Lindsey. This excellent program allows rapid comparison of spectra and enables one to do a variety of photochemically relevant calculations. |
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Absorption
This is a graph of the molar extinction coefficient of Proflavin, pH 7 dissolved in water, pH 7. It was measured by R. A. Fuh on 6/17/95 [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. Proflavin, pH 7 has a molar extinction coefficient of 38,900 M-1cm-1 at 444.75 nm [A. Albert, "Acridine syntheses and reactions. Part VI. A new dehalogenation of 9-chloroacridine and its derivatives. Further acridine ionisation constants and ultraviolet spectra.," J. Chem. Soc., 4653-4657, 1965].
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Fluorescence
This is the fluorescence emission spectrum of Proflavin, pH 7 dissolved in water, pH 7. The spectrum was taken by R. A. Fuh on 6/17/95 using an excitation wavelength of 400 nm [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. The quantum yield is 0.34 [W. H. Melhuish, "Measurement of quantum efficiencies of fluorescence and phosphorescence and some suggested luminescence standards.," J. Opt. Soc. Am., 54, 183-186, 1964]. |
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