Quinine sulfate in 0.5 M H2SO4 |
|
| The chemical structure of Quinine sulfate is shown at the right. The absorption and fluorescence emission spectra are shown below. The structure, absorption, and fluorescence data were all obtained from the PhotochemCAD package by Jonathan Lindsey. This excellent program allows rapid comparison of spectra and enables one to do a variety of photochemically relevant calculations. |
|
Absorption
This is a graph of the molar extinction coefficient of Quinine Sulfate dissolved in 0.5 M H2SO4. It was measured by R. A. Fuh on summer, 95 [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. Quinine sulfate in 0.5 M H2SO4 has a molar extinction coefficient of 5,700 M-1cm-1 at 347.5 nm [J. L. Irvin and E. M. Irvin, "A fluorometric method for the determination of pamaquine, SN-13276, and SN-3294.," J. Biol. Chem., 174, 589-596, 1948]. Quinine in acidic media has been widely recommended as a standard for static fluorescence yield determinations. Quinine is less applicable to time-resolved studies, because its fluorescence decay is not monophasic [D. Pant, U. C. Tripathi, G. C. Joshi, H. B. Tripathi, and D. D. Pant, "Photophysics of doubly-charged quinine: Steady state and time-dependent fluorescence.," J. Photochem. Photobiol. A: Chem., 51, 313-325, 1990]. |
|
Fluorescence
This is the fluorescence emission spectrum of Quinine sulfate dissolved in 0.5 M H2SO4. The spectrum was taken by R. A. Fuh on summer, 95 using an excitation wavelength of 310 nm [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. The quantum yield is 0.546 [D. F. Eaton, "Reference materials for fluorescence measurement," Pure Appl. Chem., 60, 1107-1114, 1988]. |
|