Rhodamine B

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Rhodamine B
The chemical structure of Rhodamine B is shown at the right. The absorption and fluorescence emission spectra are shown below. The structure, absorption, and fluorescence data were all obtained from the PhotochemCAD package by Jonathan Lindsey. This excellent program allows rapid comparison of spectra and enables one to do a variety of photochemically relevant calculations.
Absorption This is a graph of the molar extinction coefficient of Rhodamine B dissolved in ethanol. It was measured by R. A. Fuh on 6/20/95 [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. Rhodamine B has a molar extinction coefficient of 106,000 M^-1cm^-1 at 542.75 nm [anonymous, "Eastman Laboratory Chemicals Catalog No," 1993]. The fluorescence yield is reported to be 0.65 in basic ethanol [R. F. Kubin and A. N. Fletcher, "Fluorescence quantum yields of some rhodamine dyes.," J. Luminescence, 27, 455-462, 1982], 0.49 in ethanol [K. G. Casey and E. L. Quitevis, "Effect of solvent polarity on nonradiative processes in xanthene dyes: Rhodamine B in normal alcohols," J. Phys. Chem., 92, 6590-6594, 1988], 1.0 [R. E. Kellogg and R. G. Bennett, "Radiationless intermolecular energy transfer. III. Determination of phosphorescence efficiencies.," J. Chem. Phys., 41, 3042-3045, 1964], and 0.68 in 94% ethanol [M. J. Snare, F. E. Treloar, K. P. Ghiggino, and P. J. Thistlethwaite, "The photophysics of rhodamine B.," J. Photochem., 18, 335-346, 1982]. The fluorescence yield is temperature dependent [T. Karstens and K. Kobs, "Rhodamine B and Rhodamine 101 as reference substances for fluorescence quantum yield measurements.," J. Phys. Chem., 84, 1871-1872, 1980]. Original Data \| Extinction Data
Fluorescence This is the fluorescence emission spectrum of Rhodamine B dissolved in ethanol. The spectrum was taken by R. A. Fuh on 6/20/95 using an excitation wavelength of 510 nm [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. The quantum yield is 0.70 [F. LopezÝArbeloa, P. RuizÝOjeda, and I. LopezÝArbeloa, "Fluorescence self-quenching of the molecular forms of rhodamine B in aqueous and ethanolic solutions.," J. Luminesc., 44, 105-112, 1989]. Original Data

This page and graphs were created by Scott Prahl.