Riboflavin |
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| The chemical structure of Riboflavin is shown at the right. The absorption and fluorescence emission spectra are shown below. The structure, absorption, and fluorescence data were all obtained from the PhotochemCAD package by Jonathan Lindsey. This excellent program allows rapid comparison of spectra and enables one to do a variety of photochemically relevant calculations. |
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Absorption
This is a graph of the molar extinction coefficient of Riboflavin dissolved in ethanol. It was measured by R. A. Fuh on 6/24/97 [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. Riboflavin has a molar extinction coefficient of 33,000 M-1cm-1 at 266.5 nm [J. Koziol, "Studies on flavins in organic solvents I. Spectral characteristics of riboflavin, riboflavin tetrabutyrate and lumichrome.," Photochem. Photobiol., 5, 41-54, 1966]. See also [J. Koziol, "Studies on flavins in organic solvents I. Spectral characteristics of riboflavin, riboflavin tetrabutyrate and lumichrome.," Photochem. Photobiol., 5, 41-54, 1966]. The fluorescence yield is 0.36 in acetone and 0.37 in dioxane [J. Koziol and E. Knobloch, "The solvent effect on the fluorescence and light absorption of riboflavin and lumiflavin," Biochim. Biophys. Acta, 102, 289-300, 1965]. |
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Fluorescence
This is the fluorescence emission spectrum of Riboflavin dissolved in ethanol. The spectrum was taken by R. A. Fuh on 6/24/97 using an excitation wavelength of 450 nm [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. The quantum yield is 0.30 [M. Sun, T. A. Moore, and P.-S. Song, "Molecular luminescence studies of flavins. I. The excited states of flavins.," J. Am. Chem. Soc., 94, 1730-1740, 1972]. |
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