Uracil |
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| The chemical structure of Uracil is shown at the right. The absorption and fluorescence emission spectra are shown below. The structure, absorption, and fluorescence data were all obtained from the PhotochemCAD package by Jonathan Lindsey. This excellent program allows rapid comparison of spectra and enables one to do a variety of photochemically relevant calculations. |
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Absorption
This is a graph of the molar extinction coefficient of Uracil dissolved in water. It was measured by Junzhong Li on 12/11/97 [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. Uracil has a molar extinction coefficient of 8,200 M-1cm-1 at 258.3 nm [G. D. Fasman, Handbook of Biochemistry and Molecular Biology, Nucleic Acids, I, 65-215, CRC Press, 3 ed., 1975]. A fluorescence spectrum was not collected. The nucleic acid bases generally have very low fluorescence yields [P. R. Callis, "Polarized fluorescence and estimated lifetimes of the DNA bases at room temperature," Chem. Phys. Lett., 61, 563-567, 1979]. For fluorescence studies of the nucleic acid bases and related heterocyclic compounds, see [J. W. Longworth, R. O. Rahn, and R. G. Shulman, "Luminescence of pyrimidines, purines, nucleosides and nucleotides at 77K. The effect of ionization and tautomerization.," J. Chem. Phys, 45, 2930-2939, 1966]. |
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