These values for the molar extinction coefficient e in [cm-1/(moles/liter)] were compiled by Scott Prahl (prahl@ece.ogi.edu) using data from
To convert this data to absorbance A, multiply by the molar concentration and the pathlength. For example, if x is the number of grams per liter and a 1 cm cuvette is being used, then the absorbance is given by
(e) [(1/cm)/(moles/liter)] (x) [g/liter] (1) [cm]
A = ---------------------------------------------------
66,500 [g/mole]
using 66,500 as the gram molecular weight of hemoglobin.
To convert this data to absorption coefficient in (cm-1), multiply by the molar concentration and 2.303,
µa = (2.303) e (x g/liter)/(66,500 g Hb/mole)where x is the number of grams per liter. A typical value of x for whole blood is x=150 g Hb/liter.
lambda HbO2 Hb nm cm-1/M cm-1/M 630 680 4280 640 440 3640 650 380 3420 660 320 3200 670 320 3080 680 320 2960 690 280 2560 700 320 2160 710 340 1840 720 360 1520 730 400 1500 740 440 1520 750 520 1620 760 600 1720 770 660 1420 780 720 1120 790 760 1020 800 800 920 810 860 880 820 920 840 830 980 840 840 1040 840 850 1060 800 860 1080 840 870 1120 840 880 1160 840 890 1180 860 900 1200 880 910 1220 920 920 1240 880 930 1240 800 940 1200 800 950 1200 720